产品详情
产品名称Adipose Triglyceride Lipase Rabbit mAb
克隆号S08-3F5
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
亚型Rabbit IgG
纯化Affinity Purified
应用WB
种属反应性Human;Mouse;Rat
免疫原描述A synthetic peptide of human Adipose Triglyceride Lipase
标记Unconjugated
别名ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik
数据库入口号Swiss-Prot:Q96AD5
GeneID:57104
Uniprot
Q96AD5
Gene ID
57104
计算分子量Calculated MW: 55 kDa; Observed MW: 55 kDa
配方50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
保存Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.
应用详情
WB: 1/1000
Western blot detection of Adipose Triglyceride Lipase in K562 cell lysates using Adipose Triglyceride Lipase Rabbit mAb(1:500 diluted).Predicted band size:55kDa.Observed band size:55kDa.
All lanes : ATGL Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 5 seconds
All lanes : ATGL Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : ATGL knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Swiss-Prot Acc.Q96AD5.Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed:16239926). May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
如果您使用该产品52124发表了文章，请通知我们，让我们可以引用您的文献。
Yes
