In order to further verify phospho-specificity and ensure quality, SAB has increased phosphatase treatment method in validation of phospho-specific antibodies. Up to the present SAB has used numerous analytical methods in antibody characterization.
Western blotting(WB)
Phosphatase treatment
Stimulus treatment
Blocking peptide control
Loading control
Validation by various cell lines
Immunohistochemistry(IHC)
Blocking peptide control
Validation with different tissues
Immunofluorescence / Immunocytochemistry(IF/ICC)
Validation by various cell lines
Subcellular localization and co-localization analysis
Flow cytometry(FCM)
ELISA
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Akt(Phospho-Ser473) Antibody 11054 |
| Western blot analysis of extracts from 293 cells, treatedwith EGF or calf intestinal phosphatase (CIP), using Akt(Phospho-Ser473) Antibody #11054. | |
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PTEN(Phospho-Ser380/Thr382/Thr383) Antibody 11056 |
| Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody #11056. | |
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Tau(Phospho-Ser404) Antibody 11112 |
| Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Tau (Phospho-Ser404) Antibody #11112. | |
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NFkB-p65(Phospho-Ser529) Antibody 11217 |
| Western blot analysis of extracts from MCF cells, treated with TNFa+CA or calf intestinal phosphatase (CIP), using NFκB-p65 (Phospho-Ser529) Antibody #11217. | |
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mTOR(Phospho-Ser2448) Antibody 11221 |
| Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using mTOR (Phospho-Ser2448) Antibody #11221. | |
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