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位置: 首页 > 磷酸化抗体 > ESPL1(Phospho-S1126/801) antibody

ESPL1(Phospho-S1126/801) antibody#12175

ESPL1(Phospho-S1126/801) antibody
是否有货: yes
产品总价:
产品详情

产品名称ESPL1(Phospho-S1126/801) antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu

特异性The antibody detects endogenous level of ESPL1 only when phosphorylated at serine 1126.

免疫原类型Peptide

免疫原描述A phospho specific peptide corresponding to residues surrounding S1126 of human ESPL1.

基因/蛋白名称ESPL1

修饰Phospho

别名ESP1; SEPA

数据库入口号Swiss-Prot#: Q14674
NCBI Gene ID: 9700

UniprotQ14674

实际分子量233kd

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500 - 1:2000
Immunofluorescence: 1:20 - 1:100

Immunofluorescence analysis of U2OS cell using Phospho-ESPL1-S1126 antibody. Blue: DAPI for nuclear staining.
背景

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

Email Address

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