产品详情
产品名称DDX50 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification
应用WB, IHC
种属反应性Hu
特异性The antibody detects endogenous levels of total DDX50 protein.
免疫原类型protein
免疫原描述Fusion protein of human DDX50
基因/蛋白名称DDX50
别名GU2; GUB; mcdrh; RH-II/GuB
数据库入口号Swiss-Prot#:Q9BQ39
NCBI Gene ID:79009
Gene Accssion:BC000272
计算分子量83 kDa
浓度0.8mg/ml
配方Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
保存Store at -20C
应用详情
Western blotting:1:200-1000
Immunofluorescence:1: 25-100
The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 47041(DDX50 Antibody) at dilution 1/30. (Original magnification: ?00)
The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 47041(DDX50 Antibody) at dilution 1/30. (Original magnification: ?00)
Gel: 6%SDS-PAGE
Lysate: 40 μg, Lane 1-2: K562 and 293T cell lysates
Primary antibody:DDX50 Antibody at dilution 1/350
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 seconds
背景
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.