产品详情
产品名称Y14 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化ProA affinity purified
应用WB, ICC, IHC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名Binder of OVCA1 1 antibody
Binder of OVCA1-1 antibody
BOV 1 antibody
BOV 1A antibody
BOV 1B antibody
BOV 1C antibody
BOV-1 antibody
BOV1 antibody
BOV1A antibody
BOV1B antibody
BOV1C antibody
HSPC 114 antibody
HSPC114 antibody
MDS 014 antibody
MDS014 antibody
RBM 8 antibody
RBM 8A antibody
RBM 8B antibody
RBM8 antibody
rbm8a antibody
RBM8A_HUMAN antibody
RBM8B antibody
Ribonucleoprotein RBM 8 antibody
Ribonucleoprotein RBM 8A antibody
Ribonucleoprotein RBM8 antibody
Ribonucleoprotein RBM8A antibody
RNA binding motif protein 8 antibody
RNA binding motif protein 8A antibody
RNA binding motif protein 8B antibody
RNA binding protein 8A antibody
RNA binding protein Y14 antibody
RNA-binding motif protein 8A antibody
RNA-binding protein 8A antibody
RNA-binding protein Y14 antibody
ZNRP antibody
ZRNP 1 antibody
ZRNP1 antibody
数据库入口号Swiss-Prot#:Q9Y5S9
计算分子量20kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:2000-1:5000
IHC: 1:200
ICC: 1:200
Western blot analysis on different cell lysates using anti-Y14 rabbit polyclonal antibodies.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- Y14 rabbit polyclonal antibody.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- Y14 rabbit polyclonal antibody.
ICC staining Y14 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
背景
RNA-binding protein 8A is a protein that in humans is encoded by the RBM8A gene. This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.
背景文献
1."A novel mode of RBD-protein recognition in the Y14-Mago complex." Fribourg S., Gatfield D., Izaurralde E., Conti E. Nat. Struct. Biol. 10:433-439(2003)
2."Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex." Bono F., Ebert J., Unterholzner L., Guettler T., Izaurralde E., Conti E. EMBO Rep. 5:304-310(2004)
3."Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009)
Yes
