产品详情
产品名称YBOX1 / YB-1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Peptide affinity purified
应用WB, ICC, IHC, FC
种属反应性Hu, Ms, Rt
免疫原描述peptide
别名BP 8 antibody CBF-A antibody CCAAT binding transcription factor I subunit A antibody CCAAT-binding transcription factor I subunit A antibody CSDA2 antibody CSDB antibody DBPB antibody DNA binding protein B antibody DNA-binding protein B antibody EFI-A antibody Enhancer factor I subunit A antibody MDR NF1 antibody MGC104858 antibody MGC110976 antibody MGC117250 antibody NSEP 1 antibody NSEP1 antibody Nuclease sensitive element binding protein 1 antibody Nuclease-sensitive element-binding protein 1 antibody p50 antibody Q15905 antibody Y-box binding protein 1 antibody Y-box transcription factor antibody Y-box-binding protein 1 antibody YB 1 antibody YB-1 antibody YBOX1_HUMAN antibody YBX 1 antibody ybx1 antibody
数据库入口号Swiss-Prot#:P67809
计算分子量40kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis on mouse testis lysates using anti-YB1 rabbit polyclonal antibody.
Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-YB1 rabbit polyclonal antibody.
Immunohistochemical analysis of paraffin- embedded mouse ovary tissue using anti-YB1 rabbit polyclonal antibody.
Immunocytochemical staining of Hela cells using anti-YB1 rabbit polyclonal antibody.
Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
背景
MSY2 and YB-2 (MSY3,4) belong to the Y-box family of multifunctional proteins that regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing arginine clusters and aromatic residues. MSY2 is expressed in testis and ovary where it may repress translation of parental mRNA. The gene encoding human MSY2 maps to chromosome 17p11.2-13.1. YB-2 (MSY3,4 in mouse) is also known as DNA binding protein A and is highly expressed in the testis, heart and muscle. MSY2 and YB-2 bind to the consensus sequence 5'-UCCAUCA-3' contained in the Y-box element.
背景文献
1. Chalmey, C. et al. 2013. Systemic compensatory response to neonatal estradiol exposure does not prevent depletion of the oocyte pool in the rat. PloS one. 8: e82175. 2. Shi, K. et al. 2007. Advanced methods of isolation and identification of porcine primordial follicles. Anim Reprod Sci. 101: 163-171.
Yes
