产品详情
产品名称YAP65 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Immunogen affinity purified
应用WB, IP, IF, IHC(P)
种属反应性Hu, Ms, Rt
免疫原描述Amino acids 206-330 mapping near the C-terminus of YAP of human origin.
别名65 kDa Yes associated protein antibody 65 kDa Yes-associated protein antibody COB1 antibody YAp 1 antibody YAP 65 antibody YAP antibody YAP1 antibody YAP1_HUMAN antibody YAP2 antibody YAP65 antibody yes -associated protein delta antibody Yes associated protein 1 65kDa antibody Yes associated protein 1 antibody Yes associated protein 2 antibody yes associated protein beta antibody YKI antibody Yorkie homolog antibody
数据库入口号Swiss-Prot#:P46937
计算分子量65kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:100-1:1,000
IHC: 1:50-1:500
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
背景
The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.
背景文献
1. Yu, F.X., et al. 2012. Regulation of the Hippo-YAP pathway by G proteincoupled receptor signaling. Cell 150: 780-791. 2. Zhao, B., et al. 2012. Cell detachment activates the Hippo pathway via cytoskeleton reorganization to induce anoikis. Genes Dev. 26: 54-68.
Yes
