产品详情
产品名称E2F1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JJ092-02
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名Dmel\CG6376 antibody Dmel_CG6376 antibody drosE2F1 antibody E(Sev-CycE)3A antibody E(var)3-93E antibody E2-promoter binding facto antibody E2F 1 antibody E2F transcription factor 1 antibody E2F-1 antibody E2f-PA antibody E2f-PB antibody E2f-PC antibody E2F1 antibody E2f1 E2F transcription factor 1 antibody E2F1_HUMAN antibody Evar(3)164 antibody KIAA4009 antibody l(3)07172 antibody l(3)j3B1 antibody l(3)j3C2 antibody l(3)rM729 antibody mKIAA4009 antibody OTTHUMP00000030661 antibody PBR3 antibody PRB binding protein E2F 1 antibody PRB-binding protein E2F-1 antibody RBAP 1 antibody RBAP-1 antibody RBAP1 antibody RBBP-3 antibody RBBP3 antibody RBP 3 antibody RBP3 antibody Retinoblastoma-associated protein 1 antibody Retinoblastoma-binding protein 3 antibody Transcription factor E2F1 antibody
数据库入口号Swiss-Prot#:Q01094
计算分子量55 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.
ICC staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.
背景文献
1. Peng W & Feng J Long noncoding RNA LUNAR1 associates with cell proliferation and predicts a poor prognosis in diffuse large B-cell lymphoma. Biomed Pharmacother 77:65-71 (2016). 2. Millour J et al. ATM and p53 Regulate FOXM1 Expression via E2F in Breast Cancer Epirubicin Treatment and Resistance. Mol Cancer Ther 10:1046-58 (2011).
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