产品详情
产品名称ERCC1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JM10-07
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Hu, Ms
免疫原描述recombinant protein
别名COFS 4 antibody COFS4 antibody DNA excision repair protein ERCC 1 antibody DNA excision repair protein ERCC-1 antibody DNA excision repair protein ERCC1 antibody ERCC 1 antibody ERCC1 antibody ERCC1_HUMAN antibody Excision repair cross complementation group 1 antibody Excision repair cross complementing 1 antibody Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody Excision repair protein antibody RAD 10 antibody RAD10 antibody UV 20 antibody UV20 antibody
数据库入口号Swiss-Prot#:P07992
计算分子量36 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:100
ICC: 1:50
Western blot analysis of ERCC1 on different lysates using anti-ERCC1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Human lung Lane 3: 293T Lane 4: A549
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERCC1 antibody. Counter stained with hematoxylin.
ICC staining ERCC1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERCC1 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERCC1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERCC1 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERCC1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer; it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC-1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5' incisions during DNA repair. The ERCC1-XPF endo-nuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.
背景文献
1. Millis SZ et al. Molecular Profiling of Refractory Adrenocortical Cancers and Predictive Biomarkers to Therapy. Biomark Cancer 7:69-76 (2015). 2. Muallem MZ et al. ERCC1 expression as a predictor of resistance to platinum-based chemotherapy in primary ovarian cancer. Anticancer Res 34:393-9 (2014).