产品详情
产品名称ERp57 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JM11-44
纯化ProA affinity purified
应用WB, IP, IHC
种属反应性Hu
免疫原描述recombinant protein
别名58 kDa glucose regulated protein antibody
58 kDa glucose-regulated protein antibody
58 kDa microsomal protein antibody
Disulfide isomerase ER 60 antibody
Disulfide isomerase ER-60 antibody
Endoplasmic reticulum resident protein 57 antibody
Endoplasmic reticulum resident protein 60 antibody
ER p57 antibody
ER protein 57 antibody
ER protein 60 antibody
ERp 57 antibody
ERp57 antibody
ERp60 antibody
ERp61 antibody
Glucose Regulated Protein 58 Kd antibody
GRP 57 antibody
GRP 58 antibody
GRP57 antibody
HsT17083 antibody
p58 antibody
PDIA 3 antibody
PDIA3 antibody
PDIA3_HUMAN antibody
Phospholipase C alpha antibody
PI PLC antibody
Protein disulfide isomerase A3 antibody
Protein disulfide isomerase family A member 3 antibody
Protein disulfide-isomerase A3 antibody
数据库入口号Swiss-Prot#:P30101
计算分子量55 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1000
IHC: 1:50-1:200
IP: 1:10-1:50
Western blot analysis of ERp57 on different cells lysates using anti-ERp57 antibody at 1/500 dilution. Positive control��
Line 1: Hela
Line 2: HepG2
Line 3:293T
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERp57 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ERp57 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERp57 antibody. Counter stained with hematoxylin.
背景
Mammals defend themselves against intracellular pathogens through presentation of cytoplasmically-derived short pathogenic peptides to the cell surface of cytotoxic T lymphocytes, which subsequently leads to cytotoxic events with respect to the affected cell. Antigen presentation is mediated by major histocompatibility complex (MHC) class I molecules, which bind and coordinate short pathogenic peptides. MHC class I molecules assemble in the endoplasmic reticulum with chaperones before binding to the transporter associated with antigen processing (TAP). ERp57, also designated GRP57, GRP58, ERp60 and ERp61, is a component of the MHC class I pathway that appears to interact with MHC class I molecules before they associate with TAP. The human ERp57 gene maps to chromosome 15q15 and encodes a 505 amino acid protein. ERp57 has two Trp-Cys-Gly-His-Cys-Lys motifs completely conserved among the mammals. ERp57 may act as a protease, a protein disulfide isomerase, a phospholipase or a combination of these.
背景文献
1. He Y et al. Largescale Transcriptomics Analysis Suggests Over-Expression of BGH3, MMP9 and PDIA3 in Oral Squamous Cell Carcinoma. PLoS One 11:e0146530 (2016). 2. Kodavanti PR et al. Developmental exposure to a commercial PBDE mixture: effects on protein networks in the cerebellum and hippocampus of rats. Environ Health Perspect 123:428-36 (2015).
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