Yes
Western blot analysis of KCNAB3 expression in HEK293T (A), Hela (B), mouse kidney (C), mouse brain (D), rat kidney (E) whole cell lysates.
"Immunohistochemical analysis of KCNAB3 staining in human
breast cancer formalin fixed paraffin embedded tissue section.
The section was pre-treated using heat mediated antigen
retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and
detected using an HRP conjugated compact polymer system.
DAB was used as the chromogen. The section was then
counterstained with haematoxylin and mounted with DPX.
"Immunofluorescent analysis of KCNAB3 staining in HEK293T
cells. Formalin-fixed cells were permeabilized with 0.1% Triton
X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS
for 30 minutes at room temperature. Cells were probed with
the primary antibody in 3% BSA-PBS and incubated overnight
at 4 °C in a hidified chamber. Cells were washed with PBST and
incubated with a AF488-conjugated secondary antibody
(green) in PBS at room temperature in the dark. Phalloidin -
AF594 was used to stain Actin filaments (red). DAPI was used
to stain the cell nuclei (blue)."
