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位置: 首页 > ELISA试剂盒 > Chicken Immunoglobulin G (IgG) ELISA Kit

Chicken Immunoglobulin G (IgG) ELISA Kit#EK20118

是否有货: Yes
产品总价:
产品详情

产品名称Chicken Immunoglobulin G (IgG) ELISA Kit

简述ELISA Kit

应用ELISA

种属反应性Chicken (Gallus)

别名Immunoglobulin G,IgG,

保存The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

应用详情

Detect Range:12.5 µg/mL - 800 µg/mL

Sensitivity:6.25 µg/mL 

Sample Type:Serum, Plasma, Other biological fluids

Sample Volume: 1-200 μL

Assay Time:1-4.5h

Detection wavelength:450 nm

 

产品描述

This assay employs a two-site sandwich ELISA to quantitate IgG in samples. An antibody specific for IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

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5

注释

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  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com