产品名称Recombinant Human/Murine/Rat/Canine/Equine Brain-derived Neurotrophic Factor

来源种属Spodoptera frugiperda, Sf21 (baculovirus).

纯化> 97 % by SDS-PAGE analyses.

生物活性Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of rHu/Mu/Rt/Ca/EqBDNF is approximately 1.3 x 10³units/ug, which is calibrated against recombinant human BDNF WHO Standard. Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera is coated at 1 ug/mL, rHu/Mu/Rt/Ca/EqBDNF binds with an apparent Kd <1 nM.

内毒素Less than 0.1 EU/ug of rHu/Mu/Rt/Ca/EqBDNF as determined by LAL method.

计算分子量Apparent molecular mass of 13-14 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 119 amino acids.

序列His129-Arg247; Accession # P23560

配方Lyophilized from 0.2 μm filtered concentrated solution in 100 mM Sodium Citrate and 300 mM NaCl, pH 3.0.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.