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位置: 首页 > 磷酸化抗体 > MAP3K7 (Phospho-Thr187) Antibody

MAP3K7 (Phospho-Thr187) Antibody#11899

MAP3K7 (Phospho-Thr187) Antibody
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产品总价:
产品详情

产品名称MAP3K7 (Phospho-Thr187) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of IMAP3K7 only when phosphorylated at threonine 187.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 187 (H-M-T(p)-N-N) derived from Human MAP3K7.

基因/蛋白名称MAP3K7

修饰Phospho

别名M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7; TGF-beta- activated kinase 1; kinase TAK1

数据库入口号Swiss-Prot#: O43318;
NCBI Gene#: 6885;
NCBI Protein#: NP_003179.1

UniprotO43318

实际分子量60kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from NIH-3T3 tissue using MAP3K7 (Phospho-Thr187) antibody #11899.The lane on the right is treated with the antigen-specific peptide.
背景

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7.

Shin MS, et al. (2009)Biochim Biophys Acta 1793, 1156-64
Liu Q, Busby JC, Molkentin JD (2009)Nat Cell Biol 11, 154-61
Kim SI, Kwak JH, Wang L, Choi ME (2008)J Biol Chem 283, 10753-63

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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