免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > IKK- alpha/ beta (Phospho-Ser176/177) Antibody

IKK- alpha/ beta (Phospho-Ser176/177) Antibody#11931

IKK- alpha/ beta (Phospho-Ser176/177) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称IKK- alpha/ beta (Phospho-Ser176/177) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB,IHC,IF,ELISA

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK-alpha/beta.

基因/蛋白名称IKK- alpha/ beta

修饰Phospho

别名FLJ40509; I-kappa-B kinase; IKBKB; kinase beta; NFKBIKB

数据库入口号Swiss-Prot#: O15111/O14920;
NCBI Gene#: 1147;
NCBI Protein#: NP_001269.3

UniprotO15111/O14920

实际分子量85kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

All Lanes:IKK- alpha/ beta (Phospho-Ser176/177) Antibody at 1/ 500 dilution.Lane1:Jurkat treated with 20ng/ml TNF-α for 30min Cell lysateLane2: Huvec treated with 100ng/ml EGF for 30min Cell lysateLane3: Hela treated with 100ng/ml EGF for 30min Cell lysateLane4: HepG2 treated with 100ng/ml EGF for 30min Cell lysateLane5: Mouse Brain Tissue lysateLane6:Mouse lung Tissue lysateLane7:Rat Liver Tissue lysateLysates/proteins at 40 µg per lane. Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 85kDaObserved band size: 80kDa
背景

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.

Chandrakesan P, et al. (2010)J Biol Chem 285, 33485-98
Hinz M, et al. (2010£© Mol Cell 40, 63-74
Choudhary S, Lu M, Cui R, Brasier AR (2007)Mol Endocrinol 21, 2203-17

如果您使用该产品11931发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com