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位置: 首页 > 磷酸化抗体 > YAP (Phospho-Ser127) Antibody

YAP (Phospho-Ser127) Antibody#11952

YAP (Phospho-Ser127) Antibody
YAP (Phospho-Ser127) Antibody
YAP (Phospho-Ser127) Antibody
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产品总价:
产品详情

产品名称YAP (Phospho-Ser127) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of YAP only when phosphorylated at serine 127.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine127(A-H-S(p)-S-P) derived from Human YAP .

基因/蛋白名称YAP

修饰Phospho

别名YAP65; YAP1;

数据库入口号Swiss-Prot#: P46937;
NCBI Gene#: 10413;
NCBI Protein#: NP_001123617.1

UniprotP46937

实际分子量65kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
IF: 1:50-200 
Immunohistochemistry: 1:100 - 300

Western blot analysis of extracts from COS7 tissue using YAP (Phospho-Ser127) antibody #11952.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence analysis of Hela cell. 1,YAP (phospho Ser127) Polyclonal Antibody(red) was diluted at 1:200(4° overnight).
Immunohistochemistry analysis of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The picture on the right is blocked with the phospho peptide.
背景

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact.

Sorrentino G, et al. (2014) Nat Cell Biol 16, 357-66
Yang S, et al. (2014) Cell Signal 26, 343-51
Sun W, et al. (2014) Mol Biol Rep 41, 947-56

如果您使用该产品11952发表了文章,请通知我们,让我们可以引用您的文献。

et al,MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progressionInEMBO JOn2023 DecbyYan Sun # 1 2, Xin Jin et al..PMID: 37905571, , (2023),
PMID: 37905571
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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