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位置: 首页 > 磷酸化抗体 > FAK (Phospho-Tyr576) Antibody

FAK (Phospho-Tyr576) Antibody#11957

FAK (Phospho-Tyr576) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称FAK (Phospho-Tyr576) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Tyrosine576 (S-T-Y(p)-Y-K) derived from Human FAK.

基因/蛋白名称FAK

修饰Phospho

别名FADK 1; FAK1; Focal adhesion kinase 1; PTK2; pp125FAK

数据库入口号Swiss-Prot#: Q05397;
NCBI Gene#: 5747;
NCBI Protein#: NP_001186578.1

UniprotQ05397

实际分子量125kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from NIH-3T3 tissue using FAK (Phospho-Tyr576) antibody #11957.The lane on the right is treated with the antigen-specific peptide.
背景

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Tong L, et al. (2013)Biochim Biophys Acta 1833, 1304-15. Chen S, et al. (2013)Sci Signal 6, ra40. Tian H, et al. (2012)EMBO J 31, 3885-900.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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