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位置: 首页 > 磷酸化抗体 > IKK- gamma (Phospho-Ser31) Antibody

IKK- gamma (Phospho-Ser31) Antibody#11967

IKK- gamma (Phospho-Ser31) Antibody
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产品总价:
产品详情

产品名称IKK- gamma (Phospho-Ser31) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous level of IKK- gamma only when phosphorylated at serine 31.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 31 (E-E-S(p)-P-L) derived from Human IKK-gamma.

基因/蛋白名称IKK- gamma

修饰Phospho

别名FIP-3; FIP3; I-kappa-B kinase gamma; IKBKG; IKKAP1

数据库入口号Swiss-Prot#: Q9Y6K9;
NCBI Gene#: 8517;
NCBI Protein#: NP_001093326.2

UniprotQ9Y6K9

实际分子量48kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 293 cells treated with TNF-a using Phospho-IKK- gamma (Ser31) antibody #11967.The lane on the right is treated with the antigen-specific peptide.
背景

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly.

Carter RS, Pennington KN, Ungurait BJ, Ballard DW (2003)J Biol Chem 278, 19642-8.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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