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位置: 首页 > 磷酸化抗体 > p53 (Phospho-Ser392) Antibody

p53 (Phospho-Ser392) Antibody#11969

p53 (Phospho-Ser392) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称p53 (Phospho-Ser392) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of p53 only when phosphorylated at serine 392.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 392 (P-D-S(p)-D) derived from Human p53.

基因/蛋白名称p53

修饰Phospho

别名Antigen NY-CO-13; Cellular tumor antigen p53; Phosphoprotein p53; TP53; Tumor suppressor p53

数据库入口号Swiss-Prot#: P04637;
NCBI Gene#: 7157;
NCBI Protein#: NP_000537.3

UniprotP04637

实际分子量53kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 293 cells treated with Etoposide using Phospho-p53 (Ser392) antibody #11969.The lane on the right is treated with the antigen-specific peptide.
背景

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Choi DW, et al. (2013) Mol Cell 51, 374-85
Bagashev A, et al. (2013) Cell Cycle 12, 1569-77
Shahar OD, et al. (2013) PLoS One 8, e78472

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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