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位置: 首页 > 磷酸化抗体 > p70 S6 Kinase (Phospho-Thr389/412) Antibody

p70 S6 Kinase (Phospho-Thr389/412) Antibody#11974

p70 S6 Kinase (Phospho-Thr389/412) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称p70 S6 Kinase (Phospho-Thr389/412) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at tyrosine 389/tyrosine 412.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine389/412(G-F-T(p)-Y-V) derived from Human p70 S6 Kinase .

基因/蛋白名称p70 S6 Kinase

修饰Phospho

别名KS6B1; P70-S6K; RPS6KB1; Ribosomal protein S6 kinase; polypeptide 1

数据库入口号Swiss-Prot#: P23443;
NCBI Gene#: 6198;
NCBI Protein#: NP_001258971.1

UniprotP23443

实际分子量70kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Jurkat cells treated with Insulin using Phospho-p70 S6 Kinase (Thr389/412) antibody #11974.The lane on the right is treated with the antigen-specific peptide.
背景

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Hong S, et al. (2014) J Biol Chem
Puustinen P, et al. (2014) CJ Cell Biol 204, 713-27
Martineau Y, et al. (2014) Mol Cell Biol 34, 1046-53

如果您使用该产品11974发表了文章,请通知我们,让我们可以引用您的文献。

et al,5-HT 2 receptor mediates high-fat diet-induced hepatic steatosis and very low density lipoprotein overproduction in rats.In Obes Res Clin Pract. On 2018 Jan - Feb by Li X, Guo K et al..PMID: 27133527, , (2018),
PMID: 27133527
et al,EBV-LMP1 Regulating AKT/mTOR Signaling Pathway and WWOX in Nasopharyngeal Carcinoma.In Int J Clin Exp Pathol on 2017 Aug 1 by Lingyan Qin, Xiaohong Li,et al..PMID: 31966718, , (2017),
PMID: 31966718
et al, miR-223 reverses the resistance of EGFR-TKIs through IGF1R/PI3K/Akt signaling pathway.In Int J Oncol.On 2016 May by J Han, F Zhao et al..PMID: 26936292, , (2016),
PMID: 26936292
et al,Long-term Stress with Hyperglucocorticoidemia-induced Hepatic Steatosis with VLDL Overproduction Is Dependent on both 5-HT2 Receptor and 5-HT Synthesis in Liver.In Int J Biol Sci on 2016 Jan 1 by Jihua Fu , Shaoxin Ma et al..PMID: 26884719, , (2016),
PMID: 26884719
et al,Effective treatment with combination of peripheral 5鈥恏ydroxytryptamine synthetic inhibitor and 5鈥恏ydroxytryptamine 2 receptor antagonist on glucocorticoid鈥恑nduced whole鈥恇ody insulin resistance with hyperglycemia.In J Diabetes Investig on 2016 Nov by Shaoxin Ma , Tao Li et al..PMID:27177506, , (2016),
PMID: 27177506
et al,Rapamycin enhances the anti-cancer effect of dasatinib by suppressing Src/PI3K/mTOR pathway in NSCLC cells. In PLoS One on 2015 Jun 10 by Bin Chen, Xin Xu,et al..PMID:26061184, , (2015),
PMID: 26061184
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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