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位置: 首页 > 磷酸化抗体 > CHOP (Phospho-Ser30) Antibody

CHOP (Phospho-Ser30) Antibody#11976

CHOP (Phospho-Ser30) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称CHOP (Phospho-Ser30) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of CHOP only when phosphorylated at serine 30.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine30(V-L-S(p)-S-D) derived from Human CHOP .

基因/蛋白名称CHOP

修饰Phospho

别名C/EBP-homologous protein; DDIT3; GADD153; DNA-damage inducible transcript 3;

数据库入口号Swiss-Prot#: P35638;
NCBI Gene#: 1649;
NCBI Protein#: NP_001181982.1

UniprotP35638

实际分子量19kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Jurkat cells treated with PMA using Phospho-CHOP (Ser30) antibody #11976.The lane on the right is treated with the antigen-specific peptide.
背景

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L.

Ubeda M, Habener JF (2003) J Biol Chem 278, 40514-20

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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