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位置: 首页 > 磷酸化抗体 > BTK (Phospho-Tyr223) Antibody

BTK (Phospho-Tyr223) Antibody#11647

BTK (Phospho-Tyr223) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称BTK (Phospho-Tyr223) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous levels of BTK only when phosphorylated at tyrosine 223.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 223 (A-L-Y(p)-D-Y) derived from Human BTK.

基因/蛋白名称BTK

修饰Phospho

别名AGMX1; ATK; kinase Btk; BPK;

数据库入口号Swiss-Prot#: Q06187;
NCBI Gene#: 695;
NCBI Protein#: NP_000052.1.

UniprotQ06187

实际分子量80kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from Hela cells treated with serum using BTK (phospho-Tyr223) Antibody #11647.The lane on the right is treated with the antigen-specific peptide.
背景

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK.

Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671 - 4676.
Yan M. Li, Cancer Res., Nov 2003; 63: 7630 - 7633.
Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443 - 449.
Feng Dong, Infect. Immun., Mar 2005; 73: 1861 - 1864.
Hiroo Ueno, Mol. Biol. Cell, Feb 2

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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