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位置: 首页 > 磷酸化抗体 > SF1 (Phospho-Ser82) Antibody

SF1 (Phospho-Ser82) Antibody#11669

SF1 (Phospho-Ser82) Antibody
SF1 (Phospho-Ser82) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称SF1 (Phospho-Ser82) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from Human SF1.

基因/蛋白名称SF1

修饰Phospho

别名 BBP; CW17; SF01; ZFM1; ZNF162

数据库入口号Swiss-Prot#: Q15637;
NCBI Gene#: 7536;
NCBI Protein#: NP_004621.2.

UniprotQ15637

实际分子量68kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HUVEC cells treated with anisomycin using SF1 (Phospho-Ser82) Antibody #11669.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody #11669 (left)or the same antibody preincubated with blocking peptide (right).
背景

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Susan Y. Park, Development, May 2005; 132: 2415 - 2423.
Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986.
Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839.
Philipp Gut,Development, Oct 2005; 132: 4611 - 4619.

如果您使用该产品11669发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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