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位置: 首页 > 磷酸化抗体 > PKC α (Phospho-Tyr658) Antibody

PKC α (Phospho-Tyr658) Antibody#11684

PKC α (Phospho-Tyr658) Antibody
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产品总价:
产品详情

产品名称PKC α (Phospho-Tyr658) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of PKC α only when phosphorylated at tyrosine 658.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 658(F-S-Y(p)-V-N) derived from Human PKC α.

基因/蛋白名称PKC α

修饰Phospho

别名PKC-alpha; KPCA; PKC III; PKC-A;

数据库入口号Swiss-Prot#: P17252;
NCBI Gene#: 5578;
NCBI Protein#: NP_002728.1.

UniprotP17252

实际分子量80kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from COLO205 cells using PKC α (Phospho-Tyr658) Antibody #11684.The lane on the right is treated with the antigen-specific peptide.
背景

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells.

Finkenzeller G., Nucleic Acids Res. 18:2183-2183(1990).
McSwine-Kennick R.L., J. Biol. Chem. 266:15135-15143(1991).
Gevaert K., Nat. Biotechnol. 21:566-569(2003)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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