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位置: 首页 > 磷酸化抗体 > C/EBP-ε (Phospho-Thr74) Antibody

C/EBP-ε (Phospho-Thr74) Antibody#11686

C/EBP-ε (Phospho-Thr74) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称C/EBP-ε (Phospho-Thr74) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of C/EBP-ε only when phosphorylated at threonine 74

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 174 (L-A-T(p)-A-A) derived from Human C/EBP-ε.

基因/蛋白名称C/EBP-ε

修饰Phospho

别名CEBPE; C/EBP epsilon; CCAAT/enhancer binding protein epsilon;

数据库入口号Swiss-Prot#: Q15744;
NCBI Gene#: 1053;
NCBI Protein#: NP_001796.2.

UniprotQ15744

实际分子量34kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HUVEC cells treated with UV using C/EBP-ε (Phospho-Thr74) Antibody #11686.The lane on the right is treated with the antigen-specific peptide.
背景

C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000.
Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745 - 10751.
Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125 - 133.
Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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