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位置: 首页 > 磷酸化抗体 > EPHA2/3/4 (Phospho-Tyr588/596) Antibody

EPHA2/3/4 (Phospho-Tyr588/596) Antibody#11690

EPHA2/3/4 (Phospho-Tyr588/596) Antibody
EPHA2/3/4 (Phospho-Tyr588/596) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称EPHA2/3/4 (Phospho-Tyr588/596) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu Ms

特异性The antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y(p)-V-R)/(R-T-Y(p)-V-D) derived from Human EPHA2/3/4.

基因/蛋白名称EPHA2/3/4

修饰Phospho

别名ECK; MPK-5; SEK2; kinase EphA2;

数据库入口号Swiss-Prot#: P29317/P29320/P54764;
NCBI Gene#: 1969/2042/2043;
NCBI Protein#: NP_004422.2.

UniprotP29317/P29320/P54764

实际分子量130kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody #11690.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody #11690.
背景

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990).
Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005).
Zhuang G., J. Biol. Chem. 282:2683-2694(2007)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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