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位置: 首页 > 磷酸化抗体 > hnRPD (Phospho-Ser83) Antibody

hnRPD (Phospho-Ser83) Antibody#11696

hnRPD (Phospho-Ser83) Antibody
hnRPD (Phospho-Ser83) Antibody
hnRPD (Phospho-Ser83) Antibody
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产品总价:
产品详情

产品名称hnRPD (Phospho-Ser83) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms

特异性The antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 83(N-S-S(p)-P-R) derived from Human hnRPD .

基因/蛋白名称hnRPD

修饰Phospho

别名ROD; AUF1; HNRPD;

数据库入口号Swiss-Prot#: Q14103;
NCBI Gene#: 3184;
NCBI Protein#: NP_112738.1.

UniprotQ14103

实际分子量38kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody #11696. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody #11696 (left)or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed HeLa cells using hnRPD (Phospho-Ser83) Antibody #11696.
背景

Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Kajita Y., J. Biol. Chem. 270:22167-22175(1995).
Dempsey L.A., Genomics 49:378-384(1998).
Tolnay M., Biochem. J. 338:417-425(1999).
Grosset C., Cell 103:29-40(2000)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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