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位置: 首页 > 磷酸化抗体 > ITGB4 (Phospho-Tyr1510) Antibody

ITGB4 (Phospho-Tyr1510) Antibody#11698

ITGB4 (Phospho-Tyr1510) Antibody
ITGB4 (Phospho-Tyr1510) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称ITGB4 (Phospho-Tyr1510) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of ITGB4 only when phosphorylated at tyrosine 1510.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine1510(R-D-Y(p)-S-T) derived from Human ITGB4 .

基因/蛋白名称ITGB4

修饰Phospho

别名GP150; ITB4; CD104 antigen;

数据库入口号Swiss-Prot#: P16144;
NCBI Gene#: 3691;
NCBI Protein#: NP_000204.3.

UniprotP16144

实际分子量202kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ITGB4 (Phospho-Tyr1510) antibody #11698 (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HepG2 cells treated with Na2VO3 using ITGB4 (Phospho-Tyr1510) Antibody #11698.The lane on the right is treated with the antigen-specific peptide.
背景

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility.

Suzuki S., EMBO J. 9:757-763(1990).
Hogervorst F., EMBO J. 9:765-770(1990).
Tamura R.N., J. Cell Biol. 111:1593-1604(1990).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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