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位置: 首页 > 磷酸化抗体 > PAK1 (Phospho-Ser199) Antibody

PAK1 (Phospho-Ser199) Antibody#11699

PAK1 (Phospho-Ser199) Antibody
PAK1 (Phospho-Ser199) Antibody
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产品总价:
产品详情

产品名称PAK1 (Phospho-Ser199) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of PAK1/2 only when phosphorylated at serine 199.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 199(T-K-S(p)-V-I) derived from Human PAK1.

基因/蛋白名称PAK1

修饰Phospho

别名ADRB2; PAK 1; P65-PAK; P68-PAK;

数据库入口号Swiss-Prot#: Q13153;
NCBI Gene#: 5058;
NCBI Protein#: NP_002567.3.

UniprotQ13153

实际分子量61kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from LOVO cells treated with starved using PAK1/2 (Phospho-Ser199) Antibody #11699.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody #11699 (left)or the same antibody preincubated with blocking peptide (right).
背景

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Brown J.L., Curr. Biol. 6:598-605(1996).
Sells M.A., Curr. Biol. 7:202-210(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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