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位置: 首页 > 磷酸化抗体 > MDM2 (Phospho-Ser186/Ser188) Antibody

MDM2 (Phospho-Ser186/Ser188) Antibody#11702

MDM2 (Phospho-Ser186/Ser188) Antibody
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产品总价:
产品详情

产品名称MDM2 (Phospho-Ser186/Ser188) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IF

种属反应性Hu

特异性The antibody detects endogenous levels of MDM2 only when phosphorylated at serine 186 and serine 188.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Ser186/Ser188(H-K-S(p)-D-S(p)-I-S) derived from Human MDM2.

基因/蛋白名称MDM2

修饰Phospho

别名Oncoprotein Mdm2; p53-binding protein Mdm2; Ubiquitin-protein ligase E3 Mdm2;

数据库入口号Swiss-Prot#: Q00987;
NCBI Gene#: 4193;
NCBI Protein#: NP_001138811.1.

UniprotQ00987

实际分子量55kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunofluorescence: 1:100~1:200

Immunofluorescence staining of methanol-fixed HeLa cells using MDM2 (Phospho-Ser186/Ser188) Antibody #11702.
背景

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

M Zhou, Blood, Mar 1995; 85: 1608 - 1614.
Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030.
M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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