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位置: 首页 > 磷酸化抗体 > Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody

Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody#11703

Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody
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产品总价:
产品详情

产品名称Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at serine 304.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 304(R-S-S(p)-I-R) derived from Human Neutrophil Cytosol Factor 1.

基因/蛋白名称Neutrophil Cytosol Factor 1

修饰Phospho

别名NCF1; NCF-47K; P47 phox;

数据库入口号Swiss-Prot#: P14598;
NCBI Gene#: 653361;
NCBI Protein#: NP_000256.4.

UniprotP14598

实际分子量44kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody #11703. The lane on the left is treated with antigen-specific peptide.
背景

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).
Gorlach A., J. Clin. Invest. 100:1907-1918(1997).
Chanock S.J., Blood Cells Mol. Dis. 26:37-46(2000)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

Email Address

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