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位置: 首页 > 磷酸化抗体 > CD227/MUC1 (Phospho-Tyr1229) Antibody

CD227/MUC1 (Phospho-Tyr1229) Antibody#11707

CD227/MUC1 (Phospho-Tyr1229) Antibody
CD227/MUC1 (Phospho-Tyr1229) Antibody
CD227/MUC1 (Phospho-Tyr1229) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称CD227/MUC1 (Phospho-Tyr1229) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y(p)-E-K) derived from Human CD227/MUC1.

基因/蛋白名称CD227/MUC1

修饰Phospho

别名PUM; EMA; H23AG; MUC-1; PEM

数据库入口号Swiss-Prot#: P15941;
NCBI Gene#: 4582;
NCBI Protein#: NP_001018016.1.

UniprotP15941

实际分子量170kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody #11707.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody #11707 (left)or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody #11707.
背景

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

Lan M.S., J. Biol. Chem. 265:15294-15299(1990).
Ligtenberg M.J.L., J. Biol. Chem. 265:5573-5578(1990).
Gendler S.J., J. Biol. Chem. 265:15286-15293(1990)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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