产品名称eNOS (Phospho-Thr495) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of eNOS only when phosphorylated at threonine 495.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 495 (K-K-T(p)-F-K derived from Human eNOS .

基因/蛋白名称eNOS

修饰Phospho

别名cNOS; EC-NOS; ECNOS; NOS; NOS3

数据库入口号Swiss-Prot#: P29474;
NCBI Gene#: 4846;
NCBI Protein#: NP_000594.2.

UniprotP29474

实际分子量140kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

Western blotting: 1:500~1:1000