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位置: 首页 > 磷酸化抗体 > HSF1 (Phospho-Thr142) Antibody

HSF1 (Phospho-Thr142) Antibody#11715

HSF1 (Phospho-Thr142) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称HSF1 (Phospho-Thr142) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of HSF1 only when phosphorylated at threonine 142.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 142(L-L-T(p)-D-V) derived from Human HSF1 .

基因/蛋白名称HSF1

修饰Phospho

别名HSF 1; HSTF1; Heat shock factor protein 1;

数据库入口号Swiss-Prot#: Q00613;
NCBI Gene#: 3297;
NCBI Protein#: NP_005517.1.

UniprotQ00613

实际分子量57kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using HSF1 (Phospho-Thr142) antibody #11715 (left)or the same antibody preincubated with blocking peptide (right).
背景

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Rabindran S.K., Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910(1991).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Schuetz T.J.,Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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