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位置: 首页 > 磷酸化抗体 > PDK1 (Phospho-Tyr9) Antibody

PDK1 (Phospho-Tyr9) Antibody#11719

PDK1 (Phospho-Tyr9) Antibody
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产品总价:
产品详情

产品名称PDK1 (Phospho-Tyr9) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu

特异性The antibody detects endogenous levels of PDK1 only when phosphorylated at tyrosine 9.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 9(A-L-Y(p)-D-A) derived from Human PDK1.

基因/蛋白名称PDK1

修饰Phospho

别名hPDK1; PDPK1; mPDK1; PkB kinase;

数据库入口号Swiss-Prot#: O15530;
NCBI Gene#: 5170;
NCBI Protein#: NP_002604.1.

UniprotO15530

实际分子量63kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody #11719 (left)or the same antibody preincubated with blocking peptide (right).
背景

Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.

Alessi D.R., Curr. Biol. 7:261-269(1997).
Alessi D.R.,Curr. Biol. 7:776-789(1997).
Stephens L.R.,Science 279:710-714(1998)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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