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位置: 首页 > 磷酸化抗体 > STMN1 (Phospho-Ser63) Antibody

STMN1 (Phospho-Ser63) Antibody#11722

STMN1 (Phospho-Ser63) Antibody
STMN1 (Phospho-Ser63) Antibody
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产品总价:
产品详情

产品名称STMN1 (Phospho-Ser63) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of STMN1 only when phosphorylated at serine 63.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 63(R-K-S(p)-H-E) derived from Human STMN1.

基因/蛋白名称STMN1

修饰Phospho

别名LAG; LAP18; pp19; PR22; STN1

数据库入口号Swiss-Prot#: P16949;
NCBI Gene#: 3925;
NCBI Protein#: NP_005554.1.

UniprotP16949

实际分子量22kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STMN1 (Phospho-Ser63) antibody #11722 (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from COS cells treated with nocodazole using STMN1 (Phospho-Ser63) Antibody #.The lane on the right is treated with the antigen-specific peptide.
背景

This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Alternatively spliced transcript variants encoding the same protein have been identified.

Zhu X.-X., J. Biol. Chem. 264:14556-14560(1989).
Maucuer A., FEBS Lett. 264:275-278(1990).
Melhem R.F., J. Biol. Chem. 266:17747-17753(1991).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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