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位置: 首页 > 磷酸化抗体 > ABL1 (Phospho-Thr735) Antibody

ABL1 (Phospho-Thr735) Antibody#11725

ABL1 (Phospho-Thr735) Antibody
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产品总价:
产品详情

产品名称ABL1 (Phospho-Thr735) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of ABL1 only when phosphorylated at threonine 735.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine735 (S-V-T(p)-L-P) derived from Human ABL1.

基因/蛋白名称ABL1

修饰Phospho

别名Abelson murine leukemia viral oncogene 1; ABL1; c-ABL; JTK7; p150

数据库入口号Swiss-Prot#: P00519;
NCBI Gene#: 25;
NCBI Protein#: NP_005148.2.

UniprotP00519

实际分子量135kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from COS cells treated with EGF using ABL1 (Phospho-Thr735) Antibody #11725.The lane on the right is treated with the antigen-specific peptide.
背景

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.

Fainstein E., Oncogene 4:1477-1481(1989).
Shtivelman E., Cell 47:277-284(1986).
Chissoe S.L., Genomics 27:67-82(1995).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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