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位置: 首页 > 磷酸化抗体 > ALK (Phospho-Tyr1096) Antibody

ALK (Phospho-Tyr1096) Antibody#11726

ALK (Phospho-Tyr1096) Antibody
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产品总价:
产品详情

产品名称ALK (Phospho-Tyr1096) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu

特异性The antibody detects endogenous levels of ALK only when phosphorylated at tyrosine 1096.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 1096 (P-N-Y(p)-C-F) derived from Human ALK.

基因/蛋白名称ALK

修饰Phospho

别名ALK tyrosine kinase receptor; anaplastic lymphoma kinase; anaplastic lymphoma kinase (Ki-1); CD246; EC 2.7.10.1; kinase ALK

数据库入口号Swiss-Prot#: Q9UM73;
NCBI Gene#: 238;
NCBI Protein#: NP_004295.2

UniprotQ9UM73

实际分子量176kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue, using ALK (Phospho-Tyr1096) antibody #11726 (left)or the same antibody preincubated with blocking peptide (right).
背景

The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain.

Morris S.W., Oncogene 14:2175-2188(1997).
Morris S.W., Oncogene 15:2883-2883(1997).
Iwahara T., Oncogene 14:439-449(1997).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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