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位置: 首页 > 磷酸化抗体 > BLK (Phospho-Tyr501) Antibody

BLK (Phospho-Tyr501) Antibody#11727

BLK (Phospho-Tyr501) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称BLK (Phospho-Tyr501) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu

特异性The antibody detects endogenous levels of BLK only when phosphorylated at tyrosine 501.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 501 (R-Q-Y(p)-E-L) derived from Human BLK.

基因/蛋白名称BLK

修饰Phospho

别名 p55-BLK; EC 2.7.10.2; kinase Blk; B lymphocyte kinase; Tyrosine-protein kinase BLK

数据库入口号Swiss-Prot#: P51451;
NCBI Gene#: 640;
NCBI Protein#: NP_001706.2.

UniprotP51451

实际分子量57kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody #11727 (left)or the same antibody preincubated with blocking peptide (right).
背景

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor.

Islam K.B., J. Immunol. 154:1265-1272(1995).
Drebin J.A.,Oncogene 10:477-486(1995).
Greenman C.,Nature 446:153-158(2007).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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