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位置: 首页 > 磷酸化抗体 > CK-1α (Phospho-Tyr294) Antibody

CK-1α (Phospho-Tyr294) Antibody#11728

CK-1α (Phospho-Tyr294) Antibody
CK-1α (Phospho-Tyr294) Antibody
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产品总价:
产品详情

产品名称CK-1α (Phospho-Tyr294) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of CK-1α only when phosphorylated at tyrosine 294.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1α.

基因/蛋白名称CK-1α

修饰Phospho

别名KC1A; CKI-alpha; CSNK1A1; alpha isoform; Casein kinase I

数据库入口号Swiss-Prot#: P48729/Q8N752;
NCBI Gene#: 1452/122011;
NCBI Protein#: NP_001883.4.

UniprotP48729/Q8N752

实际分子量37kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HT-29 cells treated with heat shock using CK-1α (Phospho-Tyr294) Antibody #11728.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1α (Phospho-Tyr294) antibody #11728 (left)or the same antibody preincubated with blocking peptide (right).
背景

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Tapia C., FEBS Lett. 349:307-312(1994).
Fish K.J., J. Biol. Chem. 270:14875-14883(1995).
Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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