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位置: 首页 > 磷酸化抗体 > EPHA3/4/5 (Phospho-Tyr779/833) Antibody

EPHA3/4/5 (Phospho-Tyr779/833) Antibody#11729

EPHA3/4/5 (Phospho-Tyr779/833) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称EPHA3/4/5 (Phospho-Tyr779/833) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu

特异性The antibody detects endogenous levels of EPHA3/4/5 only when phosphorylated at tyrosine 779/833.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 779/833 (E-A-Y(p)-T-T)/(A-A-Y(p)-T-T) derived from Human EPHA3/4/5.

基因/蛋白名称EPHA3/4/5

修饰Phospho

别名EPA3; ETK; HEK; MEK4; REK

数据库入口号Swiss-Prot#: P29320/54764/54756;
NCBI Gene#: 2042/2043;
NCBI Protein#: NP_004429.1.

UniprotP29320/54764/54756

实际分子量110kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using EPHA3/4/5 (Phospho-Tyr779/833) antibody #11729 (left)or the same antibody preincubated with blocking peptide (right).
背景

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.

Wicks I.P., Proc. Natl. Acad. Sci. U.S.A. 89:1611-1615(1992).
Chiari R., Cancer Res. 60:4855-4863(2000).
Boyd A.W., J. Biol. Chem. 267:3262-3267(1992).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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