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位置: 首页 > 磷酸化抗体 > ILK (Phospho-Ser246) Antibody

ILK (Phospho-Ser246) Antibody#11733

ILK (Phospho-Ser246) Antibody
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产品总价:
产品详情

产品名称ILK (Phospho-Ser246) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu

特异性The antibody detects endogenous levels of ILK only when phosphorylated at serine 246.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 246(I-F-S(p)-H-P) derived from Human ILK.

基因/蛋白名称ILK

修饰Phospho

别名ILK1; p59ILK; kinase ILK;

数据库入口号Swiss-Prot#: Q13418;
NCBI Gene#: 3611;
NCBI Protein#: NP_001014794.1.

UniprotQ13418

实际分子量51kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using ILK (Phospho-Ser246) antibody #11733 (left)or the same antibody preincubated with blocking peptide (right).
背景

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Hannigan G.E., Nature 379:91-96(1996).
Janji B., Oncogene 19:3069-3077(2000).
Tadic B., Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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