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位置: 首页 > 磷酸化抗体 > MAP3K1 (Phospho-Thr1402) Antibody

MAP3K1 (Phospho-Thr1402) Antibody#11737

MAP3K1 (Phospho-Thr1402) Antibody
MAP3K1 (Phospho-Thr1402) Antibody
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产品总价:
产品详情

产品名称MAP3K1 (Phospho-Thr1402) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1400.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 1400(K-G-T(p)-G-A) derived from Human MAP3K1.

基因/蛋白名称MAP3K1

修饰Phospho

别名M3K1; MAP3K1; MAPKKK1; MEKK1;

数据库入口号Swiss-Prot#: Q13233;
NCBI Gene#: 4214;
NCBI Protein#: NP_005912.1.

UniprotQ13233

实际分子量130kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1400) antibody #11737 (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1400) Antibody #11737. The lane on the left is treated with antigen-specific peptide.
背景

Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.

Schmutz J., Nature 431:268-274(2004).
Xia Y., Genes Dev. 12:3369-3381(1998).
Vinik B.S., Mamm. Genome 6:782-783(1995)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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