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位置: 首页 > 磷酸化抗体 > MAP3K8 (Phospho-Ser400) Antibody

MAP3K8 (Phospho-Ser400) Antibody#11739

MAP3K8 (Phospho-Ser400) Antibody
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产品总价:
产品详情

产品名称MAP3K8 (Phospho-Ser400) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu

特异性The antibody detects endogenous levels of MAP3K8 only when phosphorylated at serine 400.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 400(C-Q-S(p)-L-D) derived from HumanMAP3K8.

基因/蛋白名称MAP3K8

修饰Phospho

别名C-COT;; M3K8; MAP3K8; TPL2;

数据库入口号Swiss-Prot#: P41279;
NCBI Gene#: 1326;
NCBI Protein#: NP_001231063.1.

UniprotP41279

实际分子量52kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody #11739 (left)or the same antibody preincubated with blocking peptide (right).
背景

This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1).

Miyoshi J., Mol. Cell. Biol. 11:4088-4096(1991).
Aoki M., J. Biol. Chem. 268:22723-22732(1993).
Chan A.M.,Oncogene 8:1329-1333(1993).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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