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位置: 首页 > 磷酸化抗体 > MAP2K3 (Phospho-Thr222) Antibody

MAP2K3 (Phospho-Thr222) Antibody#11741

MAP2K3 (Phospho-Thr222) Antibody
MAP2K3 (Phospho-Thr222) Antibody
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产品总价:
产品详情

产品名称MAP2K3 (Phospho-Thr222) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 222(A-K-T(p)-M-D) derived from Human MAP2K3 .

基因/蛋白名称MAP2K3

修饰Phospho

别名MAP2K3; MAPKK 3; MEK3; MP2K3; PRKMK3

数据库入口号Swiss-Prot#: P46734;
NCBI Gene#: 5606;
NCBI Protein#: NP_659731.1.

UniprotP46734

实际分子量39kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from Jurkat cells treated with serum using MAP2K3 (Phospho-Thr222) Antibody #11741.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody #11741 (left)or the same antibody preincubated with blocking peptide (right).
背景

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.

Derijard B., Science 267:682-685(1995).
Moriguchi T., J. Biol. Chem. 271:26981-26988(1996).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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