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位置: 首页 > 磷酸化抗体 > MAP2K7 (Phospho-Thr275) Antibody

MAP2K7 (Phospho-Thr275) Antibody#11743

MAP2K7 (Phospho-Thr275) Antibody
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产品总价:
产品详情

产品名称MAP2K7 (Phospho-Thr275) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of MAP2K7 only when phosphorylated at threonine 275.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 275(A-K-T(p)-R-S) derived from Human MAP2K7.

基因/蛋白名称MAP2K7

修饰Phospho

别名PRKMK7; JNKK2; MKK7; MAP2K7; MP2K7

数据库入口号Swiss-Prot#: O14733;
NCBI Gene#: 5609;
NCBI Protein#: NP_660186.1.

UniprotO14733

实际分子量43kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from cos-7 cells (Lane 2) and 3T3 cells (Lane 3), using MAP2K7 (Phospho-Thr275) Antibody #11743. The lane on the left is treated with antigen-specific peptide.
背景

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.

Wu Z.,Mol. Cell. Biol. 17:7407-7416(1997).
Lu X.,J. Biol. Chem. 272:24751-24754(1997).
The MGC Project Team;Genome Res. 14:2121-2127(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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