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位置: 首页 > 磷酸化抗体 > MLK1/2 (Phospho-Thr312/266) Antibody

MLK1/2 (Phospho-Thr312/266) Antibody#11744

MLK1/2 (Phospho-Thr312/266) Antibody
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产品总价:
产品详情

产品名称MLK1/2 (Phospho-Thr312/266) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of MLK1/2 only when phosphorylated at threonine 312/266.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 312/266(A-G-T(p)-Y-A) derived from Human MLK1/2.

基因/蛋白名称MLK1/2

修饰Phospho

别名M3K9; PRKE1; mixed-lineage protein kinase 1;

数据库入口号Swiss-Prot#: P80192/Q02779;
NCBI Gene#: 4293/4294;
NCBI Protein#: NP_001271159.1.

UniprotP80192/Q02779

实际分子量121kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody #11744 (left)or the same antibody preincubated with blocking peptide (right).
背景

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.

Durkin J.T., Biochemistry 43:16348-16355(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Dorow D.S.,Nature 446:153-158(2007).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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