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位置: 首页 > 磷酸化抗体 > RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody

RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody#11753

RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at serine 221/227/218/232.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 221/227/218/232(A-Y-S(p)-F-C) derived from Human RSK1/2/3/4.

基因/蛋白名称RSK1/2/3/4

修饰Phospho

别名KS6A1; KS6AA; MAPKAP-K1a; RSK1;

数据库入口号Swiss-Prot#: Q15418/P51812/Q15349/Q9UK32;
NCBI Gene#: 6195/6197/6196/27330;
NCBI Protein#: NP_002944.2.

UniprotQ15418/P51812/Q15349/Q9UK32

实际分子量85kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 cells (Lane 2), using RSK1/2/3/4 (Phospho-Ser221/227/218/232) Antibody #11753. The lane on the left is treated with antigen-specific peptide.
背景

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway.

Moller D.E., Am. J. Physiol. 266:C351-C359(1994).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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