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位置: 首页 > 磷酸化抗体 > TIE2 (Phospho-Tyr992) Antibody

TIE2 (Phospho-Tyr992) Antibody#11756

TIE2 (Phospho-Tyr992) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称TIE2 (Phospho-Tyr992) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr992.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 992 (E-V-Y(p)-V-K) derived from Human TIE2.

基因/蛋白名称TIE2

修饰Phospho

别名Angiopoietin-1 receptor,EC=2.7.10.1 Tunica interna endothelial cell kinase Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 hTIE2 p140 TEK CD202b TEK TIE2;

数据库入口号Swiss-Prot#: Q02763;
NCBI Gene#: 7010;
NCBI Protein#: NP_000450.2.

UniprotQ02763

实际分子量126kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 using TIE2 (Phospho-Tyr992) Antibody #11756.The lane on the right is treated with the antigen-specific peptide.
背景

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Schooley K.A., Baum P.R. Oncogene 8:663-670(1993)
Dunham I. Nature 429:369-374(2004)
Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases

如果您使用该产品11756发表了文章,请通知我们,让我们可以引用您的文献。

et al,Tie2 signalling through Erk1/2 regulates TLR4 driven inflammation.In Cell Signal. On 2018 Nov by Smith TL, Van Slyke P et al..PMID: 30077653 , , (2018),
PMID: 30077653
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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