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位置: 首页 > 磷酸化抗体 > Trk A (Phospho-Tyr757) Antibody

Trk A (Phospho-Tyr757) Antibody#11757

Trk A (Phospho-Tyr757) Antibody
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产品总价:
产品详情

产品名称Trk A (Phospho-Tyr757) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of Trk A only when phosphorylated at tyrosine 757.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 757 (E-V-Y(p)-A-I) derived from Human Trk A.

基因/蛋白名称Trk A

修饰Phospho

别名TRKA; TRK; NTRK1; p140-TrkA;

数据库入口号Swiss-Prot#: P04629;
NCBI Gene#: 4914;
NCBI Protein#: NP_002520.2.

UniprotP04629

实际分子量87kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using Trk A (Phospho-Tyr757) antibody #11757 (left)or the same antibody preincubated with blocking peptide (right).
背景

Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.

Martin-Zanca D., Mol. Cell. Biol. 9:24-33(1989).
Shelton D.L., J. Neurosci. 15:477-491(1995).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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